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The framework of the unliganded double mutant HIV-1 RT showed that Lys103Asn mutation facilitates coordination of the sodium ion with Lys101 O, Asn103 N and O(delta1), Tyr188 O(eta), and two water molecules. The formation from the binding pocket necessitates the removal on the sodium ion. Even though the RT alone along with the RT/HBY 097 sophistic